DNA barcoding and its utility in commonly-used medicinal snakes / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 868-874, 2015.
Article
en Zh
| WPRIM
| ID: wpr-330219
Biblioteca responsable:
WPRO
ABSTRACT
Identification accuracy of traditional Chinese medicine is crucial for the traditional Chinese medicine research, production and application. DNA barcoding based on the mitochondrial gene coding for cytochrome c oxidase subunit I (COI), are more and more used for identification of traditional Chinese medicine. Using universal barcoding primers to sequence, we discussed the feasibility of DNA barcoding method for identification commonly-used medicinal snakes (a total of 109 samples belonging to 19 species 15 genera 6 families). The phylogenetic trees using Neighbor-joining were constructed. The results indicated that the mean content of G + C(46.5%) was lower than that of A + T (53.5%). As calculated by Kimera-2-parameter model, the mean intraspecies genetic distance of Trimeresurus albolabris, Ptyas dhumnades and Lycodon rufozonatus was greater than 2%. Further phylogenetic relationship results suggested that identification of one sample of T. albolabris was erroneous. The identification of some samples of P. dhumnades was also not correct, namely originally P. korros was identified as P. dhumnades. Factors influence on intraspecific genetic distance difference of L. rufozonatus need to be studied further. Therefore, DNA barcoding for identification of medicinal snakes is feasible, and greatly complements the morphological classification method. It is necessary to further study in identification of traditional Chinese medicine.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Filogenia
/
Serpientes
/
Datos de Secuencia Molecular
/
Clasificación
/
Proteínas de Reptiles
/
Código de Barras del ADN Taxonómico
/
Genética
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Medicina Tradicional China
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Año:
2015
Tipo del documento:
Article