The development and clinical application of papillomavirus genotyping by HPV DNA suspension array the development and clinical application of papillomavirus genotyping by HPV DNA suspension array / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 498-500, 2008.
Article
en Zh
| WPRIM
| ID: wpr-332454
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop a new platform for genotyping human papillomavirus(HPV) and to investigate its effect in clinical application.</p><p><b>METHODS</b>By combining L1 consensus PCR and multiplex hybridization using a Luminex xMAP system-based suspension array, we developed a rapid high-throughput assay,the HPV DNA suspension array (HPV-SA), capable of simultaneously typing 30 HPVs, including 18 high-risk HPV genotypes and 12 low-risk HPV genotypes. 810 clinical specimens were used to investigate the effect of HPV-SA. Veracity of the genotyping result was verified by E7 type-specific PCR-DNA sequencing.</p><p><b>RESULTS</b>Among the 810 clinical specimens, 243 were found to be HPV positive,including high-risk HPV subtypes 16, 18, 26, 31, 33, 39, 45, 51, 53, 56, 58, 59, 66, 68, 73 and 82,and low-risk HPV6, 11, 34, 54, 61, 67, 70 and 84. The sensitivity tested by standard samples was up to 10 copies of HPV DNA.</p><p><b>CONCLUSION</b>The HPV-SA developed here showed high sensitivity and specificity, suitable to be applied in clinical practice for HPV diagnosis and investigation on the prevalence of HPV sub-types.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Papillomaviridae
/
Suspensiones
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Virología
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Enfermedades del Cuello del Útero
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Reacción en Cadena de la Polimerasa
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Sensibilidad y Especificidad
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Clasificación
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Análisis de Secuencia de ADN
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Técnicas de Laboratorio Clínico
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Análisis de Secuencia por Matrices de Oligonucleótidos
Tipo de estudio:
Diagnostic_studies
Límite:
Adult
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Aged
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Female
/
Humans
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2008
Tipo del documento:
Article