Effect of mesenchymal stem cell transplantation on immunological injury of the ovary in mice / 南方医科大学学报
Journal of Southern Medical University
; (12): 825-829, 2011.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-332541
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of mesenchymal stem cell (MSC) transplantation in repairing ovarian injury in mice sensitized with porcine ovarian proteins.</p><p><b>METHODS</b>Wild-type female mice with ICR background (6-8 weeks old) were divided randomly into groups A, B and C (n=12). In groups B and C, the mice were treated with the total protein extract from porcine ovary to induce immunological injury of the ovary, while those in group A received no treatment. MSCs-derived from GFP transgenic mice were transplanted into the mice of group C, and equal volume of PBS was injected intraperitoneally in mice of the other two groups. PCR was used to detect GFP gene in the genomic DNA of the ovaries to assess MSCs homing in the ovary, and the reparative effect of MSCs on ovarian injury was evaluated using HE staining and TUNEL analysis.</p><p><b>RESULTS</b>After transplantation, the MSCs could reach the injured ovaries to promote the repair of the ovarian injury, resulting also in reduced apoptosis of the granulosa cells (GCs) in the injured ovaries.</p><p><b>CONCLUSION</b>MSCs transplantation can promote the recovery of the immunological injury of the ovary in mice, the mechanism of which may involve reduced apoptosis of the GCs.</p>
Texto completo:
Disponible
Contexto en salud:
ODS3 - Meta 3.4 Reducir las muertes prematuras por enfermedades no transmisibles
Problema de salud:
Enfermedades del Sistema Endocrino
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
Enfermedades del Ovario
/
Ovario
/
Patología
/
Cirugía General
/
Células de la Médula Ósea
/
Apoptosis
/
Biología Celular
/
Trasplante de Células Madre Mesenquimatosas
/
Células Madre Mesenquimatosas
/
Células de la Granulosa
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Southern Medical University
Año:
2011
Tipo del documento:
Artículo