Genetic characterization and correlation among fragments of HN gene of the field Newcastle disease viruses / 病毒学报
Chinese Journal of Virology
; (6): 39-45, 2007.
Article
en Zh
| WPRIM
| ID: wpr-334912
Biblioteca responsable:
WPRO
ABSTRACT
Twenty-four isolates of Newcastle disease virus (NDV) prevailing during 1997 -- 2005 in China were collected. These isolates were purified by CEF plaque assay and replicated in SPF chicken embryos. The hemagglutinin-neuraminidase (HN) genes of these viruses were cloned and sequenced. The HN gene sequences of thirty-six NDV reference strains in GenBank were also used in this study. The amino acid homologing of these viruses were compared and analyzed. The correlations among different fragments of HN gene were also analyzed. The results indicated that the homology of Chinese field NDV strains was 94.4%-99.4%, but 86.9%-89% compared with LaSota and Clone30, 87.9%-89.9% to F48E9, and 87.2%-96.2% to foreign NDV strains. There had the nearest distances among Chinese NDV isolates as compared with that of the LaSota, Clone30 and F48E9 by the phylogenetic tree. However, the distances of seven foreign NDV isolates were very close to Chinese NDV isolates as compared with these of the other foreign NDV isolates. We also found that all the Chinese field isolates were devoid of glycosylation site in position 538 -- 540. There were good correlations between different length amino acid fragments and the genomes of HN, especially the 5'-terminus first 80aa.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Filogenia
/
Virus de la Enfermedad de Newcastle
/
Proteína HN
/
Pollos
/
China
/
Clasificación
/
Genética
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Virology
Año:
2007
Tipo del documento:
Article