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Investigation on the effects of soluble programmed death-1 (sPD-1) enhancing anti-tumor immune response / 华中科技大学学报(医学)(英德文版)
Artículo en Inglés | WPRIM (Pacífico Occidental) | ID: wpr-336988
Biblioteca responsable: WPRO
ABSTRACT
By using semi-quantitative RT-PCR method, it was found that PD-L1 mRNA but not PD-L2 mRNA was expressed in H22 hepatoma cells and both PD-L1 and PD-L2 mRNAs were expressed in tumor tissues of tumor-bearing mice and upregulated as compared with muscle tissues in normal mice and H22 hepatoma cells. PD-L1 and PD-L2 were also expressed on the surface of the activated T cells. The soluble recombinant sPD-1 expressed from the constructed eukaryotic expression vector could enhance the lysis of tumor cells by lymphocytes stimulated specifically with antigen. The expresssion of sPD-1 by local gene therapy on the inoculation site of H22 hepatoma cells could inhibit the growth of tumor. The results of this study indicate that expression of soluble receptor of negative costimulatory molecules could reduce the inhibitory effect on T cells in tumor microenvironment and enhance the cytotoxicity of T cells on tumor cells. This possibly provides a new method of improving efficacy of tumor gene therapy.
Asunto(s)
Texto completo: Disponible Contexto en salud: ODS3 - Meta 3.4 Reducir las muertes prematuras por enfermedades no transmisibles Problema de salud: Enfermedades del Sistema Digestivo / Neoplasia Hepática Base de datos: WPRIM (Pacífico Occidental) Asunto principal: Patología / Péptidos / Bazo / ARN Mensajero / Glicoproteínas de Membrana / Células Tumorales Cultivadas / Linfocitos T / Transfección / Linfocitos T Citotóxicos / Terapia Genética Límite: Animales Idioma: Inglés Revista: Journal of Huazhong University of Science and Technology (Medical Sciences) Año: 2004 Tipo del documento: Artículo
Texto completo: Disponible Contexto en salud: ODS3 - Meta 3.4 Reducir las muertes prematuras por enfermedades no transmisibles Problema de salud: Enfermedades del Sistema Digestivo / Neoplasia Hepática Base de datos: WPRIM (Pacífico Occidental) Asunto principal: Patología / Péptidos / Bazo / ARN Mensajero / Glicoproteínas de Membrana / Células Tumorales Cultivadas / Linfocitos T / Transfección / Linfocitos T Citotóxicos / Terapia Genética Límite: Animales Idioma: Inglés Revista: Journal of Huazhong University of Science and Technology (Medical Sciences) Año: 2004 Tipo del documento: Artículo
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