Effects of curcumin on sarcoplasmic reticulum Ca2+-ATPase in rabbits with heart failure / 中华心血管病杂志
Chinese Journal of Cardiology
; (12): 369-373, 2010.
Article
en Zh
| WPRIM
| ID: wpr-341214
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of curcumin on sarcoplasmic reticulum Ca2+-ATPase in heart failure rabbits.</p><p><b>METHODS</b>Rabbit heart failure model was made with aortic regurgitation and abdominal aorta constriction and 40 rabbits were randomly divided into 4 groups including: (1) heart failure treated with curcumin; (2) heart failure treated with placebo; (3) healthy control treated with curcumin and (4) healthy control treated with placebo. All rabbits were administrated with curcumin capsules or placebo capsules 100 mg x kg(-1) x d(-1), respectively. All groups were sacrificed after eight weeks. Myocardial ultrastructural organization was detected by transmission electron microscope. RT-PCR and Western blot were used to measure the expression of sarcoplasmic reticulum Ca2+-ATPase in mRNA and protein levels, respectively. Malachite green colorimetric assay was used to evaluate the activity of sarcoplasmic reticulum Ca2+-ATPase.</p><p><b>RESULTS</b>All detected parameters were similar between control curcumin group and control placebo group. Compared with the control groups (Groups 3 and 4), the heart/body weight ratio was significantly increased in the heart failure-curcumin group (Group 1) and the heart failure-placebo group (Group 2, all P < 0.05), but the ratio was significantly lower in heart failure-curcumin group than in heart failure-placebo group (P < 0.05). The degree of heart failure was decreased by curcumin. Activity and mRNA and protein expression for sarcoplasmic reticulum Ca2+-ATPase were significantly reduced in the heart failure-placebo group and which could be significantly attenuated by curcumin (all P < 0.05).</p><p><b>CONCLUSION</b>Curcumin could improve cardiac function via upregulating the expression of sarcoplasmic reticulum Ca2+-ATPase in this model.</p>
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
/
Retículo Sarcoplasmático
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ARN Mensajero
/
Calcio
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Curcumina
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ATPasas Transportadoras de Calcio del Retículo Sarcoplásmico
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Genética
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Insuficiencia Cardíaca
/
Metabolismo
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Cardiology
Año:
2010
Tipo del documento:
Article