Cloning and protein expression analysis of monoterpene synthase gene TwMS in Tripterygium wilfordii / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1312-1318, 2017.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-350184
Biblioteca responsable:
WPRO
ABSTRACT
In this study, we cloned a monoterpene synthases, TwMS from Tripterygium wilfordii suspension cells. TwMS gene contained a 1 797 bp open reading frame (ORF), encoding a polypeptide of 579 amino acids, which deduced isoelectric point (pI) was 6.10 and the calculated molecular weight was 69.75 kDa. Bioinformation analysis showed that the sequence of TwMS was consistent with the feature of monoterpene synthases. Differential expression analysis revealed that the relative expression level of TwMS increased significantly after being induced by methyl jasmonate (MeJA). The highest expression level occurred at 24 h. TwMS protein was successfully expressed in Escherichia coli BL21 (DE3), which laid the foundation for identifying the function of T. wilfordii monoterpene synthases.
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Journal of Chinese Materia Medica
Año:
2017
Tipo del documento:
Artículo