Identification of TPO receptors on central nervous system-a preliminary report / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 494-497, 2004.
Article
en En
| WPRIM
| ID: wpr-352034
Biblioteca responsable:
WPRO
ABSTRACT
To identify the expression of thrombopoietin (TPO) receptors (c-mpl) on central nervous system (CNS) and to evaluate the role of TPO on neural cell proliferation and protection, immunohistochemical staining, RT-PCR, MTT, and annexin-V methods were used in this study. The results showed the expression of TPO receptor on human CNS and murine neural cells. C-mpl mRNA was identified in human cerebral hemispheres and cerebellum, and mouse neural cell line C17.2 by RT-PCR. C-mpl was also confirmed in human cerebral hemispheres by immunohistostaining with con-focal microscopy. Furthermore, TPO had a stimulating effect on the growth of in vitro neural cell C17.2 by MTT assay. The anti-apoptotic effect of TPO on C17.2 cells was also demonstrated by staining with annexin-V and PI. In conclusion, the first evidence showed the expression of TPO receptor c-mpl in central nervous system. Moreover, the effect of TPO on neural cell proliferation and anti-apoptosis was also demonstrated on in vitro neural cells.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Farmacología
/
Trombopoyetina
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Química Encefálica
/
Línea Celular
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Proteínas Proto-Oncogénicas
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Eritropoyetina
/
Apoptosis
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Proteínas Oncogénicas
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Receptores de Citocinas
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Proliferación Celular
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Journal of Experimental Hematology
Año:
2004
Tipo del documento:
Article