Screening and identification of natural antisense transcript in mouse cerebral cortex / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
; (6): 620-623, 2011.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-352976
Biblioteca responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To screen and identify the possible existence of natural antisense transcript (NAT) within the mouse neocortex.</p><p><b>METHODS</b>Sixty-three cerebral cortex layer-specific genes were screened by bioinformatics prediction in mice, among which 31 mice with potential NATs were screened. NAT was identified using reverse transcription polymerase chain reaction (RT-PCR) and then cloned in pGEM-T Vector System for sequencing.</p><p><b>RESULTS</b>Among 31 genes predicted using bioinformatics, 8 were proved to be NAT positive by RT-PCR.</p><p><b>CONCLUSIONS</b>NATs exist in the mouse neocortex tissue during the development of cerebral cortex. NATs may influence mouse cortical development by regulating the related coding genes.</p>
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
ARN Mensajero
/
Datos de Secuencia Molecular
/
Línea Celular
/
Corteza Cerebral
/
ARN sin Sentido
/
Genética
Tipo de estudio:
Estudio diagnóstico
/
Estudio de tamizaje
Límite:
Animales
Idioma:
Chino
Revista:
Acta Academiae Medicinae Sinicae
Año:
2011
Tipo del documento:
Artículo