Genome amplification and sequence analysis of human bocavirus 2 / 病毒学报
Chinese Journal of Virology
; (6): 257-262, 2014.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-356606
Biblioteca responsable:
WPRO
ABSTRACT
To obtain the genome sequence of human bocavirus 2 (HBoV2), different regions of HBoV2 genome were amplified through PCR in fecal specimens which had been identified as single-positive for HBoV2 in 2010. A genome sequence of HBoV2 (HBoV2-NC, 5444 bp) was obtained after sequence assembly. The phylogenetic analysis showed that HBoV2-NC had the closest evolutionary relationship with HBoV2 Lanzhou strain. The predication of inverted terminal repeats of HBoV2-NC by DINAMelt showed that inverted terminal repeats were contained in HBoV2-NC 5' terminal, which had the typical stem-loop structure in other parvoviruses. Finally, some flanking sequences of HBoV2-NC were amplified by linker-PCR.
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
Filogenia
/
Virología
/
ARN Viral
/
Datos de Secuencia Molecular
/
Secuencia de Bases
/
Química
/
Amplificación de Genes
/
Clasificación
/
Genoma Viral
/
Infecciones por Parvoviridae
Límite:
Humanos
Idioma:
Chino
Revista:
Chinese Journal of Virology
Año:
2014
Tipo del documento:
Artículo