Significance and variance of cytokines in colonic cancer patients / 肿瘤研究与临床

ABSTRACT

Objective To detect the levels of Th1 and Th2 type cytokines of peripheral CD+4 T lymphocytes in patients with colonic cancer in order to find out the significance of variance of imbalance in clinical therapy.Methods The peripheral blood lymphocytes were stimulated by stimulator in order to enhance the expression of cytokines.Then a specific fluorochrome-conjugated monoclonal antibody was added to be combined with its specific antigen.Cytokines were detected by flow cytometry.Results The levels of Th1 type cytokines such as interferon-γ/(INF-γ),interleukin-2(IL-2)were significantly lower in patients of colonic cancer than those of normal controls.The levels of Th2 type cytokines such as interleukin-4(IL-4),interleukin-6(IL-6)and interleukin-10(IL-10)were higher in patients of colonic cancer than those of normal controls.Conclusion Th1/Th2 shihs in colonic cancer.It is suggested that the levels of INF-γ/IL-4 in colonic cancer may provide clinical index for the prognosis and evaluation of immunity.