Detection of anti-Sm antibodies in SLE serum by immuno-PCR / 中国免疫学杂志
Chinese Journal of Immunology
; (12): 263-265, 2001.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-410406
Biblioteca responsable:
WPRO
ABSTRACT
Objective:
To apply immuno-PCR for detection of anti-Sm antibodies.Methods:
Immuno-PCR was established for anti-Sm antibodies in SLE sera using SaHIgG-DNA probe. The experimental conditions were optomized and methological identifieation indicated obvious in crease(×107 ) in sensitivity compared with that of ELISA. The positive rate of anti-Sm antibodies was 54.9% in SLE sera, and significantly higher than that of ELISA. Intra-and inter-CV were 2.3%~4.8% and 3.7%~6.9% respectively. Immuno-PCR was positively correlated with ELISA( p <0.001). Therefore, immuno-PCR for anti-Sm antibodies is specific,sensitive,reproducible and practical clinically.
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio diagnóstico
Idioma:
Chino
Revista:
Chinese Journal of Immunology
Año:
2001
Tipo del documento:
Artículo