Effect of Ganoderma polysacchride on intracellular free calcium and intracellular pH in murine T cells / 中国药理学通报
Chinese Pharmacological Bulletin
; (12): 167-170, 2001.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-411509
Biblioteca responsable:
WPRO
ABSTRACT
AIM To extend the approach of the act ion of ganoderma polysacchride on intracellular signal events in T cells. METHODS Laser scanning confocal microscope imaging of the calcium and p H fluorescent indicator dye Fluo-3/AM and SNARF-1/AM were used to determine th e kinetic changes of [Ca2+]i and [pH]i in murine T cells induced b y a ganoderma polysacchride,designated GLB7. RESULTS It was fou nd that GLB7(20 mg*L-1) could increase [Ca2+]i and [pH]i at 1 min were 334.7%±16.4%(n=3)、171.6%±10.4%(n=3) respectively. T he increase in [Ca2+]i induced by GLB7 was due to the influx of extr acellular Ca2+ and intracellular Ca2+ release through both IP3-se nsitive and IP3-insensitive Ca2+ stores, and increase in [pH]i indu ced by GLB7 was relative to Na+/H+ exchange systems and [Ca2+]i. GLB 7 did not influence [Ca2+]i and [pH]i in murine T cells induced by Con A(3 mg*L-1). CONCLUSION Stimulation of the increase in [Ca2+]i and [pH]i may be an important channel for gano derma polysacchrides to achieve their pharmacological actions.
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Pharmacological Bulletin
Año:
2001
Tipo del documento:
Artículo