Cloning and prokaryotic expression of the ompA gene of Chlamydia psittaci in cows / 中国人兽共患病学报
Chinese Journal of Zoonoses
; (12): 140-143, 2010.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-433120
Biblioteca responsable:
WPRO
ABSTRACT
The ompA gene of Chlamyia psittaci in cows was amplified by PCR with primers designed based on those reported in GenBank.The amplified ompA gene was inserted into the bacterial plasmid vector pGEX-4T-1 and then transformed into E.coli BL21(DE3) with IPTG induction. The gene was derived from plasmid pMD18-T vector and then sequenced.It was demonstrated that this recombinant fusion protein of approximately 68kD in molecular mass was highly expressed in inclusion body and more pure proteins would be produced after purification.The fusion protein specifically reacted with positive sera of bovine Chlamydia as demonstrated by Western blotting. These results indicate that this recombinant fusion protein shows good reactivity and could be used to develop the diagnostic kit for bovine Chlamydia and genetic engineering vaccine.
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Zoonoses
Año:
2010
Tipo del documento:
Artículo