Involvement of the Ca2+ signaling pathway in osteoprotegerin inhibition of osteoclast differentiation and maturation
Journal of Veterinary Science
; : 151-156, 2015.
Article
en En
| WPRIM
| ID: wpr-44467
Biblioteca responsable:
WPRO
ABSTRACT
The purpose of this study was to determine whether the Ca2+ signaling pathway is involved in the ability of osteoprotegerin (OPG) to inhibit osteoclast differentiation and maturation. RAW264.7 cells were incubated with macrophage colony-stimulating factor (M-CSF) + receptor activator of nuclear factor-kappaB ligand (RANKL) to stimulate osteoclastogenesis and then treated with different concentrations of OPG, an inhibitor of osteoclast differentiation. The intracellular Ca2+ concentration [Ca2+]i and phosphorylation of Ca2+/calmodulin-dependent protein kinase II (CaMKII) in the different treatment groups were measured by flow cytometry and Western blotting, respectively. The results confirmed that M-CSF + RANKL significantly increased [Ca2+]i and CaMKII phosphorylation in osteoclasts (p < 0.01), and that these effects were subsequently decreased by OPG treatment. Exposure to specific inhibitors of the Ca2+ signaling pathway revealed that these changes varied between the different OPG treatment groups. Findings from the present study indicated that the Ca2+ signaling pathway is involved in both the regulation of osteoclastogenesis as well as inhibition of osteoclast differentiation and activation by OPG.
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Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Osteoclastos
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Diferenciación Celular
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Línea Celular
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Supervivencia Celular
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Regulación de la Expresión Génica
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Calcio
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Factor Estimulante de Colonias de Macrófagos
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Señalización del Calcio
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Ligando RANK
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Osteoprotegerina
Límite:
Animals
Idioma:
En
Revista:
Journal of Veterinary Science
Año:
2015
Tipo del documento:
Article