Influence of high-fat diet in intestinal flora and fecal weight in SD rats and its significance / 吉林大学学报(医学版)

ABSTRACT

Objective To investigate the influence of high-fat diet in the intestinal flora,fecal weight and its water content in rats, and to clarify the effect and significance of high-fat diet in the occurrence of obesity forming. Methods Twenty Sprague-Dawley (SD)rats were randomly divided into normal diet (ND)group and high-fat diet (HF)group (n=10).The rats in ND group were fed with normal diet,and the rats in HF group were fed with diet rich in oil and fat.The fresh feces were collected separately on days 1,15,30,and 49 for analysis of weight, water content,and intestinal flora.Results On the 49th day,the wet weight and water content of feces of the rats in ND group were (6.61 ± 0.17)g and (37.07 ± 3.04)%,respectively,while those in HF group were (4.46±0.30)g and (18.04±2.23)% (P<0.05).Compared with ND group ,the fecal pellets in HF group were increased obviously from the 7th day (P<0.05).There were obvious changes in intestinal microbial populations of HF group. The counts of enterococci, bifidobacteria, Lactobacillus, Bacteroides bacteria were significantly decreased on the day 49, but the count of Escherichia coli was increased significantly (P < 0.05 ). Conclusion High-fat diet can result in decrease for weight,water content,and pellets of feces;it can change the structure of intestinal flora. As result, there is a possibility that all of changes above can promote obesity in the future.