Detection and identification of high producing huperzine A by an endophytic fungus from Huperzia serrata / 国际药学研究杂志
Journal of International Pharmaceutical Research
; (6): 1112-1116, 2016.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-509101
Biblioteca responsable:
WPRO
ABSTRACT
Objective To screen endophytic fungal strains isolated from wild Huperzia serrata which can highly produce hu?perzine A. Methods The strain producing huperzine A was identified through thin layer chromatography(TLC)and high-perfor?mance liquid chromatography(HPLC)with authentic huperzine A. The fungus was identified according to its morphological character?istics and nuclear ribosomal DNA ITS sequence. Results The strain was identified as Fusarium oxysporum NSG-1 according to its morphological characteristics and nuclear ribosomal DNA ITS sequence. The amount of huperzine A produced by the fermentation li?quor of this endophytic fungus was quantified to be 1.11 mg/100 ml by HPLC,which was higher than that of previously reported endo?phytic fungi. Conclusion Endophytic fungus producing high huperzine A can be obtained from H. serrata. The production of huper?zine A based on mutagenesis and transformation of the obtained strain may adapt to the industry production.
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio diagnóstico
Idioma:
Chino
Revista:
Journal of International Pharmaceutical Research
Año:
2016
Tipo del documento:
Artículo