Electron Microscopic Autoradiography in Inner Ear Pathological Study / 听力学及言语疾病杂志

ABSTRACT

Objective To evaluate the technique of electron microscopic autoradiography in inner ear pathology.Methods The thin sections that was labeled with the thymidine-H3(3H-TdR)were prepared.The labeled specimen were processed for electron microscopic autoradiography(EM-ARG)after treatment with emulsions,development and fixation.Results Incorporation of 3H-TdR was seen over regenerated cells in the experimental papilla in regions of hair cell loss.Conclusion This methocl gives a good localization of the label at the ultrastructural level.