Protein kinase C activity in peripheral blood Tlymphocytes and its significance in patients with allergic rhinitis / 中国耳鼻咽喉头颈外科
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-530550
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE To investigate the protein kinase C (PKC) activity in peripheral blood T lymphocytes and its significance in patients with allergic rhinitis (AR) and the relationship between PKC activity and Th2 cytokines, interleukin 4 (IL-4) and interleukin 5 (IL-5) . METHODS Twenty seasona allergic rhinitis patients and 20 normal control persons participated in the study. T lymphocytes isolated and purified from blood of each person were divided into 2 groupsstimulated with and without phorbol 12-myristate 13-acetate (PMA) . The total PKC activity was detected by non-radioactive assay. The expression of IL-4 and IL-5 protein in supernatants was measured with ELISA RESULTS PKC activity, the expression of IL-4 and IL-5 protein in supernatants in AR T lymphocyte stimulated with PMA were significantly higher than those of AR T lymphocyte stimulated without PMA and those of norma (P
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Archives of Otolaryngology-Head and Neck Surgery
Año:
2006
Tipo del documento:
Artículo