Effects of Platelet Activating Factor on Murine Splenocytes Proliferation and Production of IL-2, NKCF and CSF / 第二军医大学学报

ABSTRACT

Platelet activating factor (PAF) is a biologically active membrane phospholipid mediator. In this study we investigated the effects of PAF on the lymphocyte proliferation, production of IL-2, natural killer cytotoxic factor (NKCF) and colony stimulating factor (CSF) by BALB/c mice splenocytes in vitro. The results showed that PAF inhibited lymphocyte proliferation at concentrations of 10-10~ 10-7 mol/L. CTLL-2, YAC-1 and munne bone marrow cells were used for the assays of IL-2, NKCF and CSF, respectively. Production of IL-2, NKCF and CSF from ConA stimulated murine splenocytes was significantly inhibited by PAF at 10-7 mol/L. Inhibition of spleen cells proliferation and secretion may play an important role in the pathological effects of PAF.