Antineoplastic effect of vincristine-loaded intact human erythrocytes in vitro / 解放军医学杂志
Article
en Zh
| WPRIM
| ID: wpr-556861
Biblioteca responsable:
WPRO
ABSTRACT
Objective To study the effect of Vincristine-loaded intact human erythrocytes on tumor cell in vitro. Methods VCR was loaded in intact human erythrocytes using a modified hypotonic preswelling technique. The proliferation of K562 cells after incubation with VCR-loaded erythrocytes was detected by MTT. Cellular cycle and apoptosis of K562 cells were analyzed through FACS staining with PI/Rhodamin123, and morphology of apoptotic K562 cells and their nucleus were observed through staining with Hoechest33258. Results VCR-loaded erythrocytes could efficiently inhibit the proliferation of co-cultured K562 cells and induce the cells′ apoptosis in a dosage-dependent manner. Cellular cycle analysis demonstrated that proportion of K562 cells in G 0/G 1 decreased whereas in S+G 2/M increased after incubation with VCR-loaded erythrocytes. Such effects could also be displayed from alone VCR group, and significant change was observed in K562 cells co-cultured with unloaded erythrocytes (P
Texto completo:
1
Base de datos:
WPRIM
Idioma:
Zh
Revista:
Medical Journal of Chinese People's Liberation Army
Año:
2001
Tipo del documento:
Article