Effect of gross saponins from Tribulus terrestris L on myocardial apoptosis and its related gene bcl and bax expression induced by hypoxia-reoxygenation / 解放军医学杂志

ABSTRACT

Objective To investigate the effects of gross saponins from Tribulus terrestris L (GSTT) on myocardial apoptosis, and its related gene bcl-2 and bax expression after hypoxia/reoxygenation in cultured myocardial cells of neonatal rat. Methods After myocardial hypoxia/ reoxygenation model was established by culturing primary myocardial cell of neonatal rat in vivo. The myocardial cells were divided into four groups, the sham-operated group, model group, large- and low-dose of GSTT groups. The apoptotic rate of myocardial cells was determined by flow cytometry, and the expression of bcl-2 and bax was detected by using immuno-histochemical method. Results Both large- and low- dose of GSTT could significantly decrease the apoptotic rate (P