Protective effects and machanisms of pharmacological preconditioning induced by sodium ferulate on isolated rat heart / 中国药理学通报

ABSTRACT

Aim To study the protective effects of pharmacological preconditioning induced by sodiumferulate(SF)on isolated rat heart and its machanisms.Methods Isolated SD rat hearts were divided randomly into 5 groups Control group (hearts were perfused by oxygenated perfusate for 100 minutes); Ischemia/Reperfusion(I/R) group (hearts suffered from 40 min global ischemia/30 min reperfusion after oxygenated perfusate for 30 minutes); Ischemia preconditioning group(hearts were preconditioned by 3 periods of 5-minute global ischemia/5-minute reperfusion before it suffered from I/R); SF group (previously hearts were perfused with oxygenated perfusate administered with 1.69 mmol?L-1 SF then subjected to I/R); Glibenclamide group (previously hearts were perfused with oxygenated perfusate administered with 1.69 mmol?L-1SF and 30 ?mol?L-1 Glibenclamide before it suffered from I/R). Results Compared with I/R group, 1.69 mmol?L-1 SF precondition improved significantly heart function, reduced the incidence and severity of ventricular arrhythmias, alleviated calcium overload in myocardial cell; furthered activities of Na+,K-ATPase and Ca2+-ATPase in myocardium. These effects were attenuated by 30 ?mol?L-1 Glibenclamide.Conclusions SF precondition can improve heart function and resist arrhythmia and Ca2+-overload. The cardioprotective effects of SF precondition maybe related with the opening of ATP-sensitive potassium (KATP) channels.