Development and clinical evaluation of a rapid diagnostic kit for feline leukemia virus infection
Journal of Veterinary Science
; : 91-97, 2014.
Artículo
en Inglés
| WPRIM (Pacífico Occidental)
| ID: wpr-56429
Biblioteca responsable:
WPRO
ABSTRACT
Feline leukemia virus (FeLV) causes a range of neoplastic and degenerative diseases in cats. To obtain a more sensitive and convenient diagnosis of the disease, we prepared monoclonal antibodies specific for the FeLV p27 to develop a rapid diagnostic test with enhanced sensitivity and specificity. Among these antibodies, we identified two clones (hybridomas 8F8B5 and 8G7D1) that specifically bound to FeLV and were very suitable for a diagnostic kit. The affinity constants for 8F8B5 and 8G7D1 were 0.35 x 10(9) and 0.86 x 10(9), respectively. To investigate the diagnostic abilities of the rapid kit using these antibodies, we performed several clinical studies. Assessment of analytical sensitivity revealed that the detection threshold of the rapid diagnostic test was 2 ng/mL for recombinant p27 and 12.5 x 10(4) IU/mL for FeLV. When evaluating 252 cat sera samples, the kit was found to have a kappa value of 0.88 compared to polymerase chain reaction (PCR), indicating a significant correlation between data from the rapid diagnostic test and PCR. Sensitivity and specificity of the kit were 95.2% (20/21) and 98.5% (257/261), respectively. Our results demonstrated that the rapid diagnostic test would be a suitable diagnostic tool for the rapid detection of FeLV infection in cats.
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
Productos del Gen gag
/
Sensibilidad y Especificidad
/
Leucemia Felina
/
Virus de la Leucemia Felina
/
Pruebas Diagnósticas de Rutina
/
Ratones Endogámicos BALB C
/
Anticuerpos Monoclonales
Tipo de estudio:
Estudio diagnóstico
/
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Journal of Veterinary Science
Año:
2014
Tipo del documento:
Artículo