Emulsified isoflurane protects hypoxia/reoxgenation injury in cultured neonatal rat myocardial cells by suppressing caspase-3 expression / 第三军医大学学报

ABSTRACT

Objective To investigate the protective effects of emulsified isoflurane(8% v/v) on myocardial cells after hypoxia/reoxygenation(H/R) in cultured myocardial cells from neonatal rats.Methods Myocardial hypoxia/reoxygenation model was established by culturing myocardial cells primarily isolated from neonatal rats.The myocardial cells were divided into 4 groups,the normal group,model group,fat milk treatment group,and mulsified isoflurane treatment group(0.28 mmol/L).Cellular morphologic changes and pulsation frequency were observed under inverted microscope.Cell activity was determined by XTT assay.The activities of plasma superoxide dismutase(SOD) was measured by xanthine oxidase method and the contents of serum malonicaldehyde(MDA) by thiobarbituric acid method.The activities of lactate dehydrogenase(LDH) in the culture medium after H/R injury was measured.Western blot analysis was used to detect caspase-3 protein expression in every group.Results Compared with normal group,SOD activity was decreased,and MDA content,LDH activity and the expression of caspase-3 protein were increased in model group(P