Effect and potential mechanism of replenishing the kidney and eliminating phlegm therapy on CaMKⅡ-? activities of AD rat model / 中华中医药杂志

ABSTRACT

Objective:To observe the effect of replenishing the kidney and eliminating phlegm therapy on calciumlcalmodulin-dependent protein kinase Ⅱ(CaMKⅡ-?) activities of AD rat model,and explore the potential mechanism.Methods:AD model was established by injection of ibotenic acid(IBO) and 25-35 ?-amyloid peptide(A?25-35) into basal nucleus of Meynert.After injecting for two weeks,Bushen Huatan Decoction was administered by oral gavage of high,middle and low dosages.Huperzine A-Zhulin Antun tablets served as control drug.Normal group,model group and blank group were administered with isovolumetric saline water.After treating for four weeks,hybridization in situ was used to observe the change of CaMK Ⅱ in hippocamp.Results:The expression of CaMKⅡ-? in model group was more than that in normal group(P