Regulation of the telomerase activity by recombinant human tumor necrosis factor in vitro / 中华消化杂志
Chinese Journal of Digestion
; (12)2001.
Article
en Zh
| WPRIM
| ID: wpr-569773
Biblioteca responsable:
WPRO
ABSTRACT
Objective To investigate the effect of recombinant human tumor necrosis factor (rhTNF) on telomerase activity in hepatoma cell line HepG2 cells and HepG1 6 cells. Methods TRAP ELISA method was used to determine the telomerase activity in HepG2 and HepG1 6 cells which were treated by different concentrations of rhTNF; plasmid which had been inserted 800 bp of the hTERT promoter was transiently transfected into HepG2 cells by Lipofect, and different concentrations of rhTNF were added into culture media 2 hours later, then the activity of the hTERT promoter was tested 48 hours after transfection. Results The telomerase activity of HepG2 was suppressed by rhTNF in a dose dependent manner. The expression of hTERT promoter was inhibited linearly with the dose of rhTNF within the range of 10~1 000 IU/ml. Conclusions Above results suggest that the anti tumor activity of rhTNF may attribute to its inhibitory effect on hTERT promoter expression.
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Base de datos:
WPRIM
Idioma:
Zh
Revista:
Chinese Journal of Digestion
Año:
2001
Tipo del documento:
Article