THE STUDY ON THE EFFECTS OF HMBA ALONE OR COMBINED WITH HMBPA ON MELDS19 CELL GROWTH AND DIFFERENTIATION / 解剖学报

ABSTRACT

Objective To investigate the effects of hexamethylene bisacetamide(HMBA),3-(2-hydroxy-3-methyl-butyrylamino-)-propionic acid(HMBPA) and HMBA plus HMBPA on MELDS19 cell growth and differentiation.Methods To make the MELDS19 cell growth curve,count the MELDS19 cell mitosis index,numbers of colony forming and benziding staining positivity percentage when MELDS19 cells exposed to HMBA,HMBPA and HMBA plus HMBPA.Results The numbers of the MELDS19 cells exposed to 2mmol/L HMBA was 5.0?10~5/ml,to 1?mol/L HMBPA was 6.0?10~5/ml and to 1?mol/L HMBPA+2mmol/L HMBA was 3.3?10~5/ml respectively,mitosis index and colong forming decreased.Benzidine staining positivity percentage exposed to 2mmol/L HMBA was 38%,to 1?mol/L HMBPA was 9% and to 1?mol/L HMBPA+2mmol/L HMBA was 64% respectively.Conclusion HMBA and HMBPA can inhibit MELDS19 cell proliferation and induce differentiation much effectively than HMBA or HMBPA used alone.