Development of excitotoxicity model with primary hippocampal neurons and implications of viability essay / 中华检验医学杂志
Chinese Journal of Laboratory Medicine
; (12)2003.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-584219
Biblioteca responsable:
WPRO
ABSTRACT
Objective To study the injury and protective action of drugs on neurons, the model of glutamate excitotoxicity on primary cultured hippocampual neurons from new born rats were( set up. Methods)By use of trypan blue dye staning and testing the lactate dehydrogenase leakage from cultured neurons, to investigate the neuron survival rate. Results We found the injury of neurons was related with the concentration of glutamate. NMDAR non-competitive antagonist —MK-801 could protect the glutamate excitotoxic damage on neurons. Conclusion The glutamate results in neuron injury through NMDAR; the model of neuron culture was sufficient for glutamate-induced excitotoxicity.
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Laboratory Medicine
Año:
2003
Tipo del documento:
Artículo