Practical Algorisms for PCR-RFLP-Based Genotyping of Echinococcus granulosus Sensu Lato
The Korean Journal of Parasitology
; : 679-684, 2017.
Artículo
en Inglés
| WPRIM (Pacífico Occidental)
| ID: wpr-58755
Biblioteca responsable:
WPRO
ABSTRACT
Echinococcus granulosus sensu lato (s.l.) is a causative agent of cystic echinococcosis or cystic hydatid disease in humans and domestic and wild animals. The disease is a serious health problem in countries associated with poverty and poor hygiene practices, particularly in livestock raising. We introduced a practical algorism for genotyping the parasite, which may be useful to many developing countries. To evaluate the efficiency of the algorism, we genotyped 3 unknown strains isolated from human patients. We found that unknowns 1 and 3 were included in G1, G2, and G3 genotypes group and unknown 2 was included in G4 genotype (Echinococcus equinus) according to the algorisms. We confirmed these results by sequencing the 3 unknown isolates cox1 and nad1 PCR products. In conclusion, these new algorisms are very fast genotype identification tools that are suitable for evaluating E. granulosus s.l. isolated from livestock or livestock holders, particularly in developing countries.
Texto completo:
Disponible
Contexto en salud:
Enfermedades Desatendidas
Problema de salud:
Helmintiasis
/
Enfermedades Desatendidas
/
Zoonosis
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
Parásitos
/
Pobreza
/
Reacción en Cadena de la Polimerasa
/
Higiene
/
Echinococcus granulosus
/
Países en Desarrollo
/
Equinococosis
/
Echinococcus
/
Ganado
/
Genotipo
Aspecto:
Determinantes sociales de la salud
Límite:
Animales
/
Humanos
Idioma:
Inglés
Revista:
The Korean Journal of Parasitology
Año:
2017
Tipo del documento:
Artículo