In vitro culture and identification of synovial cell of rabbit knee osteoarthritis / 国际生物医学工程杂志

ABSTRACT

Objective To replicate experimental animal model of knee osteoarthritis and to investigate the method of culture and biological characteristics of rats synoviocytes of gonarthritis in vitro.MethodsAnimal models of knee osteoarthritis were made by the Modified Hulth method.4 weeks after the replicating experiment,synovial tissues were mechanically isolated and enzyme-digested and the growth of the synovial cells was investigated.Results The synovial tissues were obviously hyperplasia in the model made by the Modified Hulth method.The synovial cells were abundant after enzyme-digested cultivation and the cell activity was higher than 98%.Conclusion The study exhibits that the Modified Hulth method apparently promotes the hyperplasia of synovial tissues.The methods of isolation and cultivation of the synovial cells in vitro is proved to be simple and feasible.