Analysis of the relationship between IL-10 SNPs and pulmonary tuberculosis susceptibility / 中国医师杂志

ABSTRACT

Objective To investigate the relationship between IL-10 SNPs and pulmonary tubercu-losis susceptibility.Methods Collected 104 blood samples of pulmonary tuberculosis patients as case group and 104 blood samples of healthy.To analyze genotype SNPs of IL-10-819 -1082 by genomic DNA extraction,PCR amplification and TOF mass spectrometry.Finally the differences of gene and the risk of pulmonary tuberculosis occurrence were analyzed.Results IL-10-819 C/C, C/T and T/T site genotype frequencies were not different between two groups( P >0.05).IL-10-1082 G/G、G/A、A/A site genotype frequencies were not different between two groups( P >0.05).In logistic multivariate regression analysis found that:be compared with IL-10-819 C/C, the risk carrying IL-10-819 C/T, T/T group respectively of pulmonary tuberculosis were OR =0.001(95%CI =0.511~1.921), P =0.977, OR =2.116(95%CI=0.263~1.219), P =0.146, without statistical significance.Be compared with IL-10-1082 A/A, the risk carrying IL-10-1082 G/A、G/G group respectively of pulmonary tuberculosis were OR =0.113(95%CI =0.540~2.393), P =0.737, OR =1.872(95%CI =0.275~1.258), P =0.171, without statisti-cal significance.Conclusions IL-10-819 and -1082 genotype and allele distribution are not different, the mutation of genotype is not correlation with pulmonary tuberculosis.