Development and validation of analytical method for the estimation of lamivudine in rabbit plasma / 药物分析学报
Journal of Pharmaceutical Analysis
; (6): 251-257, 2011.
Article
en Zh
| WPRIM
| ID: wpr-672060
Biblioteca responsable:
WPRO
ABSTRACT
Lamivudine has been widely used in the treatment of HIV disease. A reliable, sensitive reversed phase high performance liquid chromatography (RP-HPLC) method was developed and validated for lamivudine in rabbit plasma. The method was developed on Hypersil BDS C-18 column (250 mm × 4.6 mm, 5 μm) using a mobile phase of 0.25% Triethylamine buffer (pH 3.0):acetonitrile (70:30, v/v). The efficient was monitored by UV detector at 256 nm. The total run time was 15 min with a flow rate of 1.0 mL/min. Calibration curve was linear over the concentration range of 25-2000 ng/mL. The retention times of lamivudine and internal standard (Nelfinavir) were 8.78 min and 10.86 min, respectively. The developed RP-HPLC method can be successfully applied for the quantitative pharmacokinetic parameters determination of lamivudine in rabbit model.
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1
Base de datos:
WPRIM
Idioma:
Zh
Revista:
Journal of Pharmaceutical Analysis
Año:
2011
Tipo del documento:
Article