Prediction,synthesis and characteristics of B-cell epitopes of CSFV envelope glycoprotein E2 / 中国免疫学杂志
Chinese Journal of Immunology
; (12)2001.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-675593
Biblioteca responsable:
WPRO
ABSTRACT
Objective:
Study on characteristics of two synthesizd peptides based on CSFV E2 protein.Methods:
B cell epitopes of CSFV E2 antigen were predicted using accessibility and flexibility schemes, associated with antigenicity , secondary structure and multiple sites prediction. Two antigen peptides (Pep1 and Pep2) have been designed and synthesized and their reactivety were detected with 8 McAbs and antiserum against mE2 protein, then the peptides were conjugated with BSA and immunized rabbits respectively.Results:
Both Pep1 and Pep2 could react with antiserum and McAb A11, Pep2 could interact with McAbD5 and McAbD8. Only Pep1 BSA conjugate can stimulate high level and specific antibodies.Conclusion:
The peptide1 has good antigenicity.
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio pronóstico
Idioma:
Chino
Revista:
Chinese Journal of Immunology
Año:
2001
Tipo del documento:
Artículo