FEATURES OF SURFACE CELLS OF MESENTERY UNDER SCANNING ELECTRON MICROSCOPE / 解剖学报
Acta Anatomica Sinica
; (6)1955.
Article
en Zh
| WPRIM
| ID: wpr-680629
Biblioteca responsable:
WPRO
ABSTRACT
This paper reports a simple method using the mesentery as a naturai mountingsubstrate for the study of mesenteric free cells under the scanning electron micros-cope.Mouse mesentery was cut into pieces about Ⅰ square centimeter in size andadhered on to neutral filter paper.The specimen were fixed in 5% glutaraldehyde and1% osmium tetroxide for 20 minutes,each step washed lightly with pH 7.4 PBS,dehydrated with ascending series of alcohol,put into amyl acetate,critical pointdried with CO_2,coated with gold and examined in scanning electron microscope.The morphologic features of free cells in the peritoneal lumen,such as polymorpho-nuclear leucocytes,macrophages and lymphocytes could be observed satisfactorily.We studied in particular the relationship between immunoreactive cells and targetcells(fetal liver cell)injected into the peritoneal cavity,Polymorphonuclear leuco-cytes could be seen invading into and eroding the target cells and macrophage cau-sing their swelling and lysis.Cooperative action of immunoreactive cells and anincrease in number of milk spots were also observed.
Texto completo:
1
Base de datos:
WPRIM
Idioma:
Zh
Revista:
Acta Anatomica Sinica
Año:
1955
Tipo del documento:
Article