Quantitative determination of limonin in traditional Chinese medicine by LC-MS/MS / 中成药
Chinese Traditional Patent Medicine
; (12)1992.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-681960
Biblioteca responsable:
WPRO
ABSTRACT
Objective:
To develop a simple LC MS/MS method for the measurement of limonin in fructus evodiae, cortex phellodendri, cortex dictamni and its preparation.Methods:
Separation was obtained by using a discovery C 18 column (150mm?2.1 mm), The mobile phaseAwater-0.1%HAc;Bacetonitrile-0.1%HAc;The elution program was gradient and the flow rate was 0.2mL?min -1 . The mass detector was set MS1(IS)=187.0 and MS2(Limonin)=471.0 by multiple reaction monitor(MRM) respectively.The sample was added psoralen(IS), then extracted with methanol chloroform(1∶3).Results:
Limonin was linear from 25 to 1000ng?mL -1 ( r = 0.9996), The regression equation was C=3.124 As/Ai 1.935?10 -3 . The average recovery was 100.2%.Conclusion:
This method is simple, quick, sensitive and exclusive.
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Traditional Patent Medicine
Año:
1992
Tipo del documento:
Artículo