Increasing the Production of IgG in Batch Tissue-culture with IgG-free Ascites / 中国生物工程杂志
China Biotechnology
; (12): 87-92, 2005.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-735619
Biblioteca responsable:
WPRO
ABSTRACT
An effective method has been developed for laboratory scale production of IgG. Hybridomas were cultured in serum-free media with 2% IgG-free ascites. Cell density of up to 3.55 × 10 6cells/ml and antibody concentration of 135μ g/ml after purification were abtained, which is four time more than total production of that of IgG concentration in serum-free media. This in vitro method allows great improvement in antibodies production in batch tissue culture. The method reported here is easy to handle and is economical and universally adaptable.
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
China Biotechnology
Año:
2005
Tipo del documento:
Artículo