Activation of β-catenin signaling in aggrecan-expressing cells in temporomandibular joint causes osteoarthritis-like defects / 国际口腔科学杂志·英文版
International Journal of Oral Science
; (4): 13-13, 2018.
Artículo
en Inglés
| WPRIM (Pacífico Occidental)
| ID: wpr-772302
Biblioteca responsable:
WPRO
ABSTRACT
β-Catenin plays a critical role in cartilage formation and development. To further understand the role of β-catenin in osteoarthritis (OA) development in temporomandibular joint (TMJ), we have generated β-catenin conditional activation mice (β-cat(ex3) ) by breeding Agc1-CreER mice with β-catenin mice. Results of histologic analysis showed the progressive TMJ defects in 3- and 6-month-old β-cat(ex3) mice (tamoxifen induction was performed at 2 weeks of age), including decreased chondrocyte numbers in the superficial layer associated with less Alcian blue staining, increased numbers of hypertrophic chondrocytes in deep layers, and rough articular surface. Compared to the TMJ phenotype of β-cat(ex3) mice, β-cat(ex3) mice showed much severe morphological defects in the superficial layer of TMJ. This may reflect that Agc1-CreER mice could efficiently target cells in the superficial layer of TMJ. Results of immunostaining showed significantly increased expression of MMP13, Col-X, Adamts4, and Adamts5 in TMJ of β-cat(ex3) mice. Results of proliferating cell nuclear antigen (PCNA), Ki67, and terminal deoxinucleotidyl transferase-mediated dUTP-fluorescein nick end labeling (TUNEL) staining further demonstrated that cell proliferation was decreased and cell apoptosis was increased in condylar cartilage of β-cat(ex3) mice. Our findings indicate that abnormal upregulation of β-catenin in TMJ leads to defects assembling to OA-like phenotype, further demonstrating that β-catenin plays a critical role in TMJ pathogenesis.
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
Osteoartritis
/
Fenotipo
/
Propiedades de Superficie
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Articulación Temporomandibular
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Transducción de Señal
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Cartílago Articular
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Diferenciación Celular
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Células Cultivadas
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Apoptosis
/
Etiquetado Corte-Fin in Situ
Tipo de estudio:
Estudio de etiología
/
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
International Journal of Oral Science
Año:
2018
Tipo del documento:
Artículo