Expression of Aquaporin 1 and 4 in the Choroid Plexus and Brain Parenchyma of Kaolin-Induced Hydrocephalic Rats / 대한신경손상학회지
Korean Journal of Neurotrauma
; : 68-75, 2017.
Artículo
en Inglés
| WPRIM (Pacífico Occidental)
| ID: wpr-80645
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE:
Aquaporin (AQP) is a recently discovered protein that regulates water homeostasis. The present study examines changes in AQP 1 and 4 in kaolin induced experimental hydrocephalic rats to elucidate the pathophysiology of water homeostasis in the disease.METHODS:
Hydrocephalus was induced by percutaneous intracisternal injection of kaolin. The brain parenchyma and choroid plexus were obtained at 3, 7, 14 and 30 days after injection. Protein expressions of AQP 1 and 4 were measured by western blot, immunohistochemistry (IHC) and immunofluorescence (IF) stains.RESULTS:
In the choroid plexus of the kaolin-induced hydrocephalus group, AQP 1 expression identified by western blot exhibited sharp decrease in the early stage (55% by the 3rd day and 22% by the 7th day), but indicated a 2.2-fold increase in the later stage (30th day) in comparison with control groups. In the parenchyma, a quantitative measurement of AQP 4 expression revealed variable results on the 3rd and 7th days, but indicated expression 2.1 times higher than the control in the later stage (30th day). In addition, the IHC and IF findings supported the patterns of expression of AQP 1 in the choroid plexus and AQP 4 in the parenchyma.CONCLUSION:
Expression of AQP 1 decreased sharply in the choroid plexus of acute hydrocephalus rats and increased at later stages. Expression of AQP 4 in the brain parenchyma was variable in the early stage in the hydrocephalus group, but was higher than in the control in the later stage. These findings suggest a compensating role of AQPs in water physiology in hydrocephalus.
Texto completo:
Disponible
Contexto en salud:
ODS3 - Meta 3.4 Reducir las muertes prematuras por enfermedades no transmisibles
Problema de salud:
Enfermedad de los Órganos de los Sentidos
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
Fisiología
/
Encéfalo
/
Inmunohistoquímica
/
Agua
/
Western Blotting
/
Coroides
/
Plexo Coroideo
/
Técnica del Anticuerpo Fluorescente
/
Acuaporinas
/
Colorantes
Tipo de estudio:
Estudio pronóstico
Límite:
Animales
Idioma:
Inglés
Revista:
Korean Journal of Neurotrauma
Año:
2017
Tipo del documento:
Artículo