Development of a double antibody sandwich ELISA for detection of Chikungunya virus antigen / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 148-152, 2017.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-808155
Biblioteca responsable:
WPRO
ABSTRACT
Objective@#To establish a method for detection of chikungunya virus(CHIKV) antigen.@*Methods@#CHIKV virus like particle(VLP), that contains all structural proteins, was prepared by baculovirus expression system. Mice and rabbits were immunized with the VLP to develop antibodies against CHIKV. A double antibody sandwich ELISA was established for detection of CHIKV antigens. The concentrations of the antibodies used and the reaction conditions were optimized. The detection limit and repeatability of the ELISA was evaluated.@*Results@#The sensitivity and specificity was estimated by 10 mimicking CHIKV sera, 90 health person sera, 40 other virus infected sera. It was show that the specificity of DAS-ELISA was 100%, the detection limit was 10 TCID50, the coefficients of variation (CV) within plate was <5%, the CV of different plates was <10%.@*Conclusions@#The double antibody sandwich ELISA established in this study can be used to detect the CHIKV antigen in acute phase serum of patient and provide a method for detection of CHIKV.
Texto completo:
Disponible
Contexto en salud:
Enfermedades Desatendidas
Problema de salud:
Fiebre Chikungunya
/
Enfermedades Desatendidas
Base de datos:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio diagnóstico
Idioma:
Chino
Revista:
Chinese Journal of Experimental and Clinical Virology
Año:
2017
Tipo del documento:
Artículo