Inhibitive effect of triptolide on invasiveness of human fibrosarcoma cells by downregulating matrix metalloproteinase-9 expression
Asian Pacific Journal of Tropical Medicine
; (12): 482-485, 2011.
Article
en En
| WPRIM
| ID: wpr-820107
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To explore the molecular mechanisms of antitumor properties of triptolide, a bioactive component isolated from the Chinese herb Tripterygium wolfordii Hook F.@*METHODS@#Human fibrosarcoma HT-1080 cells were treated with different doses of triptolide for 72 h. Then the expression and activity of matrix metalloproteinase (MMP)-2 and -9 were measured and the invasiveness of triptolide-treated HT-1080 cells was compared with that of anti-MMP-9-treated HT-1080 cells.@*RESULTS@#18 nmol/L triptolide inhibited the gene expression and activity of MMP-9, but not those of MMP-2, in HT-1080 cells. In addition, both 18 nmol/L triptolide and 3 μg/mL anti-MMP-9 significantly reduced the invasive potential of HT-1080 cells, by about 50% and 35%, respectively, compared with the control. Whereas there was no significant difference between the effect of 18 nmol/L triptolide and that of anti-MMP-9 on invasive potential of HT-1080 cells.@*CONCLUSIONS@#These data suggest that triptolide inhibits tumor cell invasion partly by reducing MMP-9 gene expression and activity.
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Base de datos:
WPRIM
Asunto principal:
Patología
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Farmacología
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Fenantrenos
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Regulación hacia Abajo
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Metaloproteinasa 2 de la Matriz
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Metaloproteinasa 9 de la Matriz
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Línea Celular Tumoral
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Diterpenos
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Quimioterapia
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Compuestos Epoxi
Límite:
Humans
Idioma:
En
Revista:
Asian Pacific Journal of Tropical Medicine
Año:
2011
Tipo del documento:
Article