Preparation of high specific antibody against human C1q/TNF-related protein-1 / 国际药学研究杂志

ABSTRACT

Objective: To prepare specific antibody against human C1q/TNF-related protein-1 (hCTRP1). Methods: A C-terminal antigenic peptide of hCTRP1 was synthesized and injected into the Newzealand rabbits. The antibody was purified by affinity chromatography column. Results: High titer antisera against the hCTRP1 was prepared and the antiserum's titration was over 1: 64 000 by indirect ELISA. The specificity of the purified antibody by affinity chromatography was identified by Western blot and ELISA. Conclusion: The high titer antiserum is prepared by injection for rabbits and the specificity of the antibody purified from the antiserum is determined.