A system research on HPLC characteristic fingerprint of Pinelliae Rhizoma and Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine / 中草药

ABSTRACT

Objective: To study the characteristic fingerprint of different extract parts from Pinelliae Rhizoma (PR) and Pinelliae Rhizoma Praeparatum cum Zingibere et Alumine (PRPZA) by HPLC, to elucidate the major material foundations before and after processing, and to provide reliable method and scientific basis for the quality control of PR and PRPZA. Methods: The HPLC fingerprints of water, 75% ethanol and 95% ethanol extracts from PR and PRPZA were established. The similarity was analyzed with "Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica". The principal component analysis (PCA) was performed by SPSS software 17.0. Results: Six common modes of the HPLC characteristic fingerprint of different extract parts from PR and PRPZA have been established. Six specific peaks were identified as inosine, guanosine, adenosine, succinic acid, ephedrine hydrochloride, and 6-gingerol, respectively. Compared with the characteristic fingerprint of PR, there were two more peaks existed in retention time 18.3 (peak 10) and 73.5 min (peak 19, 6-gingerol) of PRPZA. Conclusion: It is the first time to establish the HPLC characteristic fingerprint of different extract parts from PR and PRPZA. The method is stable, time-saving, and reliable, and could provide an efficient basis for the quality control of PR and PRPZA.