Dihydromyricetin inhibits proliferation and migration of gastric cancer cells through regulating Akt/STAT3 signaling pathways and HMGB1 expression / 南方医科大学学报
Journal of Southern Medical University
; (12): 87-92, 2021.
Article
en Zh
| WPRIM
| ID: wpr-880832
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To investigate the inhibitory effects of dihydromyricetin on the proliferation and migration of gastric cancer BGC-823 cells and explore the molecular mechanisms.@*METHODS@#BGC-823 cells in routine culture were treated with different concentrations of dihydromyricetin (0, 40, 60, 80, 100, and 120 μg/mL) for 24 h, and the changes in cell viability were detected using CCK-8 assay; colony forming assay and Transwell assay were performed to assess the changes in colonyforming and migration abilities of the cells, respectively. The levels of MMP-2 and MMP-9 in the treated cells were determined using ELISA, and Western blotting was used to detect the expressions of E-cadherin, N-cadherin, cyclin D1, cyclin E1, HSP70 and HMGB1 and the phosphorylation levels of Akt and Stat3.@*RESULTS@#CCK-8 assay showed that dihydromyricetin treatment dose-dependently inhibited the viability of BGC-823 cells (@*CONCLUSIONS@#Dihydromyricetin inhibits the proliferation and migration of BGC-823 cells through suppressing the activation of Akt/stat3 signaling pathways and HMGB1 expression.
Palabras clave
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Neoplasias Gástricas
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Movimiento Celular
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Proteína HMGB1
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Línea Celular Tumoral
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Flavonoles
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Proliferación Celular
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Proteínas Proto-Oncogénicas c-akt
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Factor de Transcripción STAT3
Límite:
Humans
Idioma:
Zh
Revista:
Journal of Southern Medical University
Año:
2021
Tipo del documento:
Article