Simultaneous determination of trihexyphenidyl, chlorpromazine and clozapine in blood by GC-MS / 法医学杂志
Journal of Forensic Medicine
; (6): 271-273, 2011.
Article
en Zh
| WPRIM
| ID: wpr-983663
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To develop a method to measure trihexyphenidyl, chlorpromazine and clozapine in human blood with GC-MS.@*METHODS@#The specimens were alkalized (pH > 10) and extracted with V (benzene):V(ethyl acetate) = 1:1, and qualitatively analyzed using GC-MS-Full Scan with internal standard SKF525A. The specimens were alkalized (pH > 10) and extracted with V(benzene):V(ethyl acetate) = 1:1, and quantitatively analyzed using GC-MS-SIM with internal standard diazepam-d5.@*RESULTS@#The lowest detection limits of trihexyphenidyl, chlorpromazine and clozapine were 0.3, 0.3 and 0.7 ng/mL (S/N > or = 3) respectively. The calibration curve in 20-10 000 ng/mL showed a good linear distribution. The recovery rate was 79.9% to 85.5%. The RSDs of intraday and interday were less than 5.1%.@*CONCLUSION@#The established method was simple, sensitive and accurate for simultaneous determination of trihexyphenidyl, chlorpromazine and clozapine in human blood, and can be applied in forensic toxicological cases.
Texto completo:
1
Base de datos:
WPRIM
Asunto principal:
Solventes
/
Antipsicóticos
/
Trihexifenidilo
/
Clorpromazina
/
Reproducibilidad de los Resultados
/
Sensibilidad y Especificidad
/
Clozapina
/
Toxicología Forense
/
Concentración de Iones de Hidrógeno
/
Cromatografía de Gases y Espectrometría de Masas
Límite:
Adult
/
Female
/
Humans
/
Male
Idioma:
Zh
Revista:
Journal of Forensic Medicine
Año:
2011
Tipo del documento:
Article