Effects of ketamine on proliferation and apoptosis of pheochromocytoma cell / 法医学杂志
Journal of Forensic Medicine
; (6): 405-412, 2011.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-983688
Biblioteca responsable:
WPRO
ABSTRACT
OBJECTIVE@#To explore the effect of ketamine on adrenal pheochromocytoma (PC12) cell proliferation inhibition and induction of apoptosis and its mechanism.@*METHODS@#PC12 cells of rats were models for dopaminergic neuron. PC12 cells were cultured with ketamine at concentrations of 0.9, 1.2, 1.5, 1.8 and 2.1 mmol/L, respectively. The cell viability was measured by MTT method after incubation at 12, 24, 48 and 72h. Hoechst stain was used to observe the morphological changes of apoptosis. PC12 cells cultured after 48 h with different concentrations of ketamine were selected to detect apoptotic rate using flow cytometry and detect the expression of bax and bcl-2 proteins using Western blotting.@*RESULTS@#For different concentrations of ketamine, vitality of PC12 cells significantly decreased with increase of the incubation time. Apoptosis was obviously observed using Hoechst staining. Flow cytometry showed that apoptosis rates significantly increased with increasing ketamine concentrations.@*CONCLUSION@#Ketamine can inhibit the proliferation of PC12 cell by inducing apoptosis of the PC12 cell in a concentrations-dependent manner. The underlying mechanism may be related to promoting the expression of bax and inhibiting the expression of bcl-2 in the cells.
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Asunto principal:
Factores de Tiempo
/
Regulación de la Expresión Génica
/
Western Blotting
/
Células PC12
/
Apoptosis
/
Proteínas Proto-Oncogénicas c-bcl-2
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Proliferación Celular
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Relación Dosis-Respuesta a Droga
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Proteína X Asociada a bcl-2
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Citometría de Flujo
Límite:
Animales
Idioma:
Chino
Revista:
Journal of Forensic Medicine
Año:
2011
Tipo del documento:
Artículo