Determination of 9 components Simultaneously in Swertia chirayita by HPLC method / 国际中医中药杂志
International Journal of Traditional Chinese Medicine
; (6): 594-599, 2023.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-989662
Biblioteca responsable:
WPRO
ABSTRACT
Objective:
To establish a HPLC method for determinating 9 components simultaneously in Swertia chirayita.Methods:
By useing water Sunfire C18 column (4.6 mm× 250 mm,5 μm); Gradient elution was carried out with methanol-0.05% phosphoric acid solution as mobile phase. Setting the column temperature at 30 ℃, the flow rate at 1.0 ml/min, and the detection wavelength at 254 nm.Results:
9 components showed good linear relationship within the injection quality range. The recovery rates of wertiamarin, Gentiopicroside, Angelica glycosides,Mangiferin, Isolysine, Gentianoside, Diol glycoside, 8-hydroxy-1,3,5 trimethoxyketone, and Daisy leaf gentinone were 95.38%, 92.41%, 95.14%, 91.87%, 92.24%, 92.51%, 95.08%, 91.72%, 95.74% ( n=6).Conclusion:
The method is simple, efficient, sensitive, accurate, economical and practical, with repeatability and stability. It could provide reference for the quality control and comprehensive utilization of Swertia chirayita.
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
International Journal of Traditional Chinese Medicine
Año:
2023
Tipo del documento:
Artículo