Mechanism of Osteoking in Improving Energy Metabolism in Rats with Myofascial Pain Syndrome Based on cAMP/PKA/PGC1α Signaling Pathway / 中国实验方剂学杂志
Chinese Journal of Experimental Traditional Medical Formulae
; (24): 54-62, 2023.
Artículo
en Chino
| WPRIM (Pacífico Occidental)
| ID: wpr-998162
Biblioteca responsable:
WPRO
ABSTRACT
ObjectiveFrom the perspective of energy metabolism, the mechanism of Osteoking (OK) in the treatment of myofascial pain syndrome (MPS) was revealed through systems biology prediction combined with holistic animal experimental validation methods. MethodFirstly, the key targets of MPS and their related molecular mechanisms were predicted by the systems biology method, and the core network targets were screened. Then, the network-predicted targets were verified by animal experiments. Specifically, 60 SD rats were randomly divided into normal group, model group, low, medium, and high dose OK groups (0.66, 1.31, 2.63 mL·kg-1), and positive celecoxib group (21 mg·kg-1). The MPS model was established by beating combined with a centrifugal exercise method for eight weeks. Except for two days after modeling, the intervention of OK or celecoxib was performed. After the completion of the model, the drug was administered for two weeks. The histopathological changes of trigger point muscle tissue were observed by hematoxylin-eosin staining. The content/activity of Na-K-ATP enzyme (Na+-K+-ATPase), Ca2+ pump (Ca2+ATPase), Ca2+, lactate dehydrogenase (LDH), glutathione (GSH), malondialal (MDA), superoxide dismutase (SOD), cyclic adenosine phosphate (cAMP), and protein kinase A (PKA) in serum and/or trigger point muscle tissue in MPS rats was detected by enzyme-linked immunosorbent assay. Protein expression levels of PKA and the peroxisome proliferator-activated receptor γ coactivator 1α (PGC1α) in MPS rats were detected by immunohistochemistry. The protein expression levels of PKA, PGC1α, and mitochondrial transcription factor A (TFAM) in MPS rats were detected by Western blot. ResultThe network prediction results suggest that OK acts on the key target of energy metabolism related to the occurrence and development of MPS and may participate in the activation of the cAMP/PKA/PGC1α signaling pathway. The experimental validation results show that compared with the normal group, contracture nodules and disordered arrangement of muscle fibers appear in the trigger point muscle tissue of MPS rats. Na+-K+-ATPase, Ca2+ATPase, SOD activity, Ca2+, and GSH contents in serum and/or trigger point muscle tissue are significantly decreased (P<0.01). Both LDH activity and MDA contents are significantly increased (P<0.01), and the protein expression levels of cAMP, PKA, PGC1α, and TFAM are significantly decreased (P<0.01). Compared with the model group, OK improves the histopathological morphology of trigger point muscle fibers in MPS rats, and after the intervention of OK, Na+-K+-ATPase, Ca2+ATPase, SOD activity, Ca2+, and GSH contents in serum and/or trigger point muscle tissue in MPS rats are significantly increased (P<0.05, P<0.01). LDH activity and MDA contents are significantly reduced (P<0.05, P<0.01). The protein expression levels of cAMP, PKA, PGC1α, and TFAM are significantly increased (P<0.05, P<0.01). ConclusionThe mechanism of OK's intervention in MPS rats may be related to its effective activation of the cAMP/PKA/PGC1α signaling pathway, thus promoting mitochondrial energy metabolism and trigger point muscle fiber damage repair in muscle cells.
Texto completo:
Disponible
Base de datos:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Experimental Traditional Medical Formulae
Año:
2023
Tipo del documento:
Artículo