Rapid detection of sepsis using cesda: the caenorhabditis elegans sepsis detection assay
Rev. Soc. Bras. Med. Trop
; 52: e20180300, 2019. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-1041561
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract INTRODUCTION The nematode Caenorhabditis elegans was used as a biological sensor to detect the urine of sepsis patients (CESDA assay). METHODS C. elegans was aliquoted onto the center of assay plates and allowed to migrate towards sepsis (T) or control (C) urine samples spotted on the same plate. The number of worms found in either (T) or (C) was scored at 10-minute intervals over a 60-minute period. RESULTS The worms were able to identify the urine (<48 hours) of sepsis patients rapidly within 20 minutes (AUROC=0.67, p=0.012) and infection within 40 minutes (AUROC=0.80, p=0.016). CONCLUSIONS CESDA could be further explored for sepsis diagnosis.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Biomarcadores
/
Quimiotaxia
/
Caenorhabditis elegans
/
Sepse
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Rev. Soc. Bras. Med. Trop
Assunto da revista:
Medicina Tropical
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
Malásia
Instituição/País de afiliação:
Universiti Kebangsaan Malaysia/MY