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Beneficial effects of Cuscuta chinensis extract on glucocorticoid-induced osteoporosis through modulation of RANKL/OPG signals
Mo, Hui; Zhang, Ning; Li, Huifu; Li, Fan; Pu, Rong.
Afiliação
  • Mo, Hui; Maoming People's Hospital. Department of Nuclear Medicine. Maoming. CN
  • Zhang, Ning; Maoming People's Hospital. Department of Nuclear Medicine. Maoming. CN
  • Li, Huifu; Maoming People's Hospital. Department of Nuclear Medicine. Maoming. CN
  • Li, Fan; Maoming People's Hospital. Department of Nuclear Medicine. Maoming. CN
  • Pu, Rong; Clinical Laboratory of the Third People's Hospital of Dongguan City. Dongguan. CN
Braz. j. med. biol. res ; 52(12): e8754, 2019. tab, graf
Artigo em Inglês | LILACS | ID: biblio-1055467
Biblioteca responsável: BR1.1
ABSTRACT
Cuscuta chinensis Lam. (Convolvulaceae) is an important herbal medicine widely used to improve sexual function, treat osteoporosis, and prevent aging, and has been reported to exhibit anti-osteoporotic effects in vitro. However, the activity of Cuscuta chinensis Lam. on glucocorticoid-induced osteoporosis still remains unclear. The present study aimed to assess the protective effect and the underlying mechanism of action of Cuscuta chinensis extract (CCE) against glucocorticoid-induced osteoporosis in vivo. Sprague-Dawley rats were randomly divided into four groups as follows control group, osteoporosis group, and 2 CCE-treated osteoporosis groups (100 mg·kg-1·day-1). Blood samples and femur bones were collected for immunohistochemistry, biochemical, mRNA expression, and western blot analysis. HPLC analysis revealed that chlorogenic acid, quercetin, and hyperin were the major constituents of CCE. The results indicated that CCE increased bone length, bone weight, and bone mineral density and suppressed dexamethasone (DEX)-induced reduction in body weight. In addition, TRAP staining indicated that CCE reduced osteoclasts in DEX-induced osteoporosis rats. Mechanistically, CCE treatment alleviated the increase of bone resorption markers and the decline of osteogenic markers, which might be partially mediated by regulation of RANKL/OPG and RunX2 pathways. These results suggest that CCE showed promising effects in the protection against glucocorticoid-induced osteoporosis through protecting osteoblasts and suppressing osteoclastogenesis.
Assuntos


Texto completo: Disponível Coleções: Bases de dados internacionais Contexto em Saúde: ODS3 - Meta 3.6 Reduzir as mortes e traumatismos por acidentes de transito Problema de saúde: Abuso de Substâncias e Acidentes Rodoviários Base de dados: LILACS Assunto principal: Osteoporose / Dexametasona / Extratos Vegetais / Cuscuta / Osteoprotegerina / Glucocorticoides Limite: Animais Idioma: Inglês Revista: Braz. j. med. biol. res Assunto da revista: Biologia / Medicina Ano de publicação: 2019 Tipo de documento: Artigo País de afiliação: China Instituição/País de afiliação: Clinical Laboratory of the Third People's Hospital of Dongguan City/CN / Maoming People's Hospital/CN

Texto completo: Disponível Coleções: Bases de dados internacionais Contexto em Saúde: ODS3 - Meta 3.6 Reduzir as mortes e traumatismos por acidentes de transito Problema de saúde: Abuso de Substâncias e Acidentes Rodoviários Base de dados: LILACS Assunto principal: Osteoporose / Dexametasona / Extratos Vegetais / Cuscuta / Osteoprotegerina / Glucocorticoides Limite: Animais Idioma: Inglês Revista: Braz. j. med. biol. res Assunto da revista: Biologia / Medicina Ano de publicação: 2019 Tipo de documento: Artigo País de afiliação: China Instituição/País de afiliação: Clinical Laboratory of the Third People's Hospital of Dongguan City/CN / Maoming People's Hospital/CN
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